| Autor: |
Olga Dremova, Maximilian Mimmler, Nadja Paeslack, My Phung Khuu, Zhenling Gao, Markus Bosmann, Lucien P. Garo, Nathalie Schön, Alexa Mechler, Yunes Beneich, Vivian Rebling, Amrit Mann, Giulia Pontarollo, Klytaimnistra Kiouptsi, Christoph Reinhardt |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Frontiers in Immunology, Vol 14 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1664-3224 |
| DOI: |
10.3389/fimmu.2023.1275109 |
| Popis: |
In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions is a key requirement. Here, we review basic principles of germ-free isolator technology, the suitability of various sterilization methods, and the use of sterility testing methods to monitor germ-free mouse colonies. We also discuss their effectiveness and limitations, and share the experience with protocols used in our facility. In addition, possible sources of isolator contamination are discussed and an overview of reported contaminants is provided. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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