| Autor: |
Edgar Olguin-Maciel, Alfonso Larqué-Saavedra, Patricia E. Lappe-Oliveras, Luis F. Barahona-Pérez, Liliana Alzate-Gaviria, Rubí Chablé-Villacis, Jorge Domínguez-Maldonado, Daniella Pacheco-Catalán, Hector A. Ruíz, Raúl Tapia-Tussell |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Microorganisms, Vol 7, Iss 11, p 483 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2076-2607 |
| DOI: |
10.3390/microorganisms7110483 |
| Popis: |
Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of Trametes hirsuta (Bm-2) was evaluated for bioethanol production from Brosimum alicastrum in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of T. hirsuta Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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