| Autor: |
Soares-Silveira Alda, Roberts Morven, Fraga Lúcia, Cecchini Luiza, Dunne David, Correa-Oliveira Rodrigo, Dutra Walderez O, Webster Michelle, Yssel Hans, Gollob Kenneth J |
| Jazyk: |
angličtina |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
BMC Immunology, Vol 3, Iss 1, p 8 (2002) |
| Druh dokumentu: |
article |
| ISSN: |
1471-2172 |
| DOI: |
10.1186/1471-2172-3-8 |
| Popis: |
Abstract Background Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-γ or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP. Results Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed. Conclusions High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-γ. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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