| Autor: |
Tetsuya Kuwano, Takayuki Kanno, Minoru Tobiume, Yuichiro Hirata, Harutaka Katano, Michiko Koga, Hiroyuki Nagai, Takeya Tsutsumi, Noritada Yoshikawa, Hiroshi Yotsuyanagi, Satoshi Kutsuna, Yusuke Miyazato, Noriko Kinoshita-Iwamoto, Norio Ohmagari, Taiichiro Kobayashi, Kazuaki Fukushima, Masaru Tanaka, Akifumi Imamura, Yui Ueda, Maeko Iwamura, Naoto Takada, Takayoshi Inoue, Tetsuro Matano, Ai Kawana-Tachikawa, Tadaki Suzuki |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Scientific Reports, Vol 14, Iss 1, Pp 1-8 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2045-2322 |
| DOI: |
10.1038/s41598-024-77862-0 |
| Popis: |
Abstract There have been several reports of skin manifestations in patients with coronavirus disease 2019 (COVID-19). However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected on the skin surface, including the sebum, of these patients. In this study, SARS-CoV-2 RNA was detected using real-time reverse-transcription polymerase chain reaction (RT-PCR) assay of skin surface lipids (SSLs) collected using an oil-blotting film from the faces of hospitalized patients with COVID-19. Human transcriptome analysis was also performed using the same samples. In facial SSLs of patients with COVID-19, the RT-PCR positivity rate was 84.6% (11/13 samples) within 5 days and 30.4% (7/23 samples) by 6–10 days of symptom onset. In the transcriptome analysis, the most characteristic SSL-RNA profile was the upregulation of interferon-stimulated gene (ISG)-related genes, such as ISG15, IFITM1, and MX1. This study presents an alternative technique using SSLs for non-invasive SARS-CoV-2 RNA detection and simultaneous analysis of human molecular pathogenesis in patients with COVID-19. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
