| Autor: |
Terunobu Saito, Sen-itiroh Hakomori |
| Jazyk: |
angličtina |
| Rok vydání: |
1971 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 12, Iss 2, Pp 257-259 (1971) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)39537-7 |
| Popis: |
The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia–silica gel (Florisil) column, and (c) deacetylation of glycolipid in chloroform–methanol–sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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