Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model
| Autor: | G. P. Liao, Y. Choi, K. Vojnits, H. Xue, K. Aroom, F. Meng, H. Y. Pan, R. A. Hetz, C. J. Corkins, T. G. Hughes, F. Triolo, A. Johnson, Kenneth J. Moise, K. P. Lally, C. S. Cox, Y. Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Stem Cells International, Vol 2017 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1687-966X 1687-9678 |
| DOI: | 10.1155/2017/1764523 |
| Popis: | Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects. Control rats received decellularized diaphragm scaffolds alone. We found that the composite tissues that combined hAFMSCs demonstrated improved physiological function as well as the muscular-tendon structure, compared with the native contralateral hemidiaphragm of the same rat. Our results indicate that the decellularized diaphragm scaffolds are a potential support material for diaphragmatic hernia repair and the composite grafts with hAFMSC are able to accelerate the functional recovery of diaphragmatic hernia. |
| Databáze: | Directory of Open Access Journals |
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