Population Behavior Analysis of dspE and pelD Regulation in Erwinia chrysanthemi 3937

Autor: Quan Peng, Shihui Yang, Amy O. Charkowski, Mee-Ngan Yap, Douglas A. Steeber, Noel T. Keen, Ching-Hong Yang
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Zdroj: Molecular Plant-Microbe Interactions, Vol 19, Iss 4, Pp 451-457 (2006)
Druh dokumentu: article
ISSN: 1943-7706
0894-0282
DOI: 10.1094/MPMI-19-0451
Popis: Erwinia chrysanthemi 3937 (Ech3937) is a phytopathogenic bacterium with a wide host range. The pectinolytic enzymes secreted by the bacterium and the type III secretion system (T3SS) are essential for full virulence. We used the green fluorescent protein gene as a reporter to investigate the expression of dspE (a putative T3SS effector) and pelD (a major pectin-degrading enzyme) in populations of Ech3937 under different conditions. Gene expression was analyzed by measuring the fluorescence intensity of individual cells with a fluorescence-activated cell sorter. Ech3937 dspE was induced in minimal medium (MM) with only a portion of Ech3937 cells (43.03%) expressing dspE after 12 h of culture. The nutrient-rich King's medium B did not fully eliminate the expression of dspE; a small percentage of Ech3937 cells (5.55%) was able to express dspE after 12 h of culture in this medium. In all, 68.95% of Ech3937 cells expressed pelD after 12 h of culture in MM supplemented with polygalacturonic acid (PGA). However, 96.34% of Ech131 cells (an hrpL deletion mutant of Ech3937) expressed pelD after 12 h of culture in MM supplemented with PGA. In potato tubers, 6.32% of the bacterial cells expressed dspE 2 h after inoculation, whereas only 0.25% of the cells expressed pelD. However, after 24 h, the percentage of cells expressing pelD (68.48%) was approximately 3.5 times that of cells expressing dspE (19.39%). In contrast to potato tubers, similar proportion of Ech3937 cells expressing dspE (39.34%) and pelD (40.30%) were observed in Chinese cabbage 24 h after inoculation. From promoter activity and real-time quantitative results, the expression of pelD in Ech3937 was demonstrated to be downregulated by HrpL in MM supplemented with PGA.
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