Phylogenetic of Four Bamboo Genera Based on NdhH Sequences, Chloroplast DNA

Autor: Annisa, Hafzari Rini, Irawan Budi, Setiawati Tia, Kusmoro Joko
Jazyk: English<br />French
Rok vydání: 2021
Předmět:
Zdroj: E3S Web of Conferences, Vol 249, p 03005 (2021)
Druh dokumentu: article
ISSN: 2267-1242
DOI: 10.1051/e3sconf/202124903005
Popis: Bamboos are versatile plants with many usages. Indonesia has a high diversity of Bamboos species and has been using them for a long time. As multipurpose plants, they are vulnerable to over exploration. Much of the bamboo land has been converted into housing or agricultural land. Development and conservation need to be balanced, to protect the genetic diversity, bamboo in particular. We studied the relationship among four genera of bamboo that mostly found in Indonesia using gene that presents in chloroplast DNA, ndhH. DNA from twenty-four samples from Bambusa, Dendrocalamus, Gigantochloa and Schizostachyum were extracted and amplified using specific ndhH primer. Sequencing results were analysed with BioEdit 7.2 software. Phylogenetic tree was build using raxmlGUI 2.0 software. The result showed that ndhH gene generated good sequence quality. It has small variation among samples. The result suggested that ndhH was able to differentiate some species within Schizostachyum, however; it was not a success for others.
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