| Autor: |
M Ivanova-Kicheva, A Kukov, D Daskalova, E Solenkova, I Nikolov, L Todorov |
| Jazyk: |
Bulgarian<br />Czech<br />English<br />Croatian<br />Hungarian<br />Polish<br />Slovak |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Journal of Central European Agriculture, Vol 9, Iss 2, Pp 283-288 (2008) |
| Druh dokumentu: |
article |
| ISSN: |
1332-9049 |
| Popis: |
Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered translocation of PS in treated with dexamethazone groups at the 10-th min and in control no treated varied from 2.74%±0.65 to 2.30%±0.89. After the 5 hour of incubation these % increased to 39.83±3.33 for the treated group and 23.44±1.12 for the control. It was concluded that Annexin V binding assay is more sensitive in the detection of deterioration in membrane function than other conventional methods such as motility analysis and supravital techniques. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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