Glucose Sensor Using Sol–Gel Coating Layer Deposited on PMMA Optical Fiber: An Enzyme Activity Measurement System

Autor: Jorge-A. Ortega-Contreras, Edgar Alvarado-Méndez, Guillermo Almanza-Rodríguez, María del Carmen Hernández, Luis Celaya-García
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Gels, Vol 9, Iss 8, p 608 (2023)
Druh dokumentu: article
ISSN: 2310-2861
DOI: 10.3390/gels9080608
Popis: In the present work, a biocatalytic glucose optical sensor produced by immobilizing glucose oxidase (GOD) as a recognition molecule over a PMMA (polymethylmethacrylate) optical fiber is introduced. An enzymatic encapsulation process was carried out using the sol–gel method, depositing a TEOS-based coating by immersion at the end of an optical fiber; the biosensor was characterized using different glucose levels. Finally, the best way to encapsulate the enzyme and prevent it from degrading is to perform the process at room temperature, and later implement the deposition of the coating on the fiber. The drying process was optimal below 8 °C.
Databáze: Directory of Open Access Journals