| Autor: |
Ángel Alegría, Alberto Pintor-Cora, Daniela C. S. Ribeiro, Teresa M. López-Díaz, Jose M. Rodríguez-Calleja |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Medical Sciences Forum, Vol 24, Iss 1, p 17 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2673-9992 |
| DOI: |
10.3390/ECA2023-16400 |
| Popis: |
The proliferation of enterobacteria that produce extended spectrum β-lactamases (ESBLs) has become an important public health concern. The objective of this work was to optimize a phenotypic method for straightforward and rapid ESBL detection in strains obtained from food environments, employing MALDI-TOF mass spectrometry. A collection of 162 enterobacterial isolates, including ESBL producers, AmpC producers, ESBL-AmpC coproducers and sensitive strains was analyzed using a quick method based on cefotaxime hydrolysis. Statistical analysis showed that although the presence of constitutive AmpC hyperproducers could reduce its specificity, this MALDI-TOF assay is an excellent tool for quickly classifying ESBL-producing strains of foodborne Enterobacteriaceae. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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