| Autor: |
Amira Awadalla, Wael I. Mortada, Hassan Abol-Enein, Ahmed A. Shokeir |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Heliyon, Vol 6, Iss 12, Pp e05642- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2405-8440 |
| DOI: |
10.1016/j.heliyon.2020.e05642 |
| Popis: |
Objective: To investigate the relationship between blood levels of cadmium (Cd) and lead (Pb) and the expression of miRNA-21 among bladder cancer (BC) patients. Material and methods: The blood concentrations of Cd and Pb in 268 BC patients and 132 controls were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The blood concentrations of Cd and Pb were interpreted according to the type and stage of the carcinoma. The expression of miRNA-21 was assessed by quantitative reverse transcription polymerase chain reaction in cancerous and adjacent non-cancerous bladder tissues among the patient groups. Results: The blood concentrations of Cd and Pb were statistically elevated in BC patients compared to those of the controls. This elevation is more prevalent in groups with muscle-invasive bladder cancer (MIBC) than those with non-muscle invasive bladder cancer (NMIBC). Among the BC group, miRNA-21 was upregulated in cancerous tissues relative to adjacent non-cancerous tissues. Moreover, the expression was significantly higher in patients with MIBC compared to those with NMIBC. The expression of miRNA-21 in cancerous tissues was significantly associated with blood concentration of Cd and Pb among BC patients. Conclusion: There is a relationship between Cd and Pb body burden and the tissue expression of miRNA-21 among BC patients. This indicates the role of miRNA-21 in Cd and Pb induced BC. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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