| Autor: |
Samar Shurbaji, Mahmoud Khatib A. A. Al-Ruweidi, Fatma Hassan Ali, Fatiha M. Benslimane, Huseyin C. Yalcin |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Methods and Protocols, Vol 3, Iss 4, p 65 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2409-9279 |
| DOI: |
10.3390/mps3040065 |
| Popis: |
Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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