| Autor: |
Madhubanti Basu, Michael S. Piepenbrink, Czestochowa Francois, Fritzlaine Roche, Bo Zheng, David A. Spencer, Ann J. Hessell, Christopher F. Fucile, Alexander F. Rosenberg, Catherine A. Bunce, Jane Liesveld, Michael C. Keefer, James J. Kobie |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Cell Reports Medicine, Vol 1, Iss 2, Pp 100015- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-3791 |
| DOI: |
10.1016/j.xcrm.2020.100015 |
| Popis: |
Summary: Induction of persistent HIV-1 Envelope (Env) specific antibody (Ab) is a primary goal of HIV vaccine strategies; however, it is unclear whether HIV Env immunization in humans induces bone marrow plasma cells, the presumed source of long-lived systemic Ab. To define the features of Env-specific plasma cells after vaccination, samples were obtained from HVTN 105, a phase I trial testing the same gp120 protein immunogen, AIDSVAX B/E, used in RV144, along with a DNA immunogen in various prime and boost strategies. Boosting regimens that included AIDSVAX B/E induced robust peripheral blood plasmablast responses. The Env-specific immunoglobulin repertoire of the plasmablasts is dominated by VH1 gene usage and targeting of the V3 region. Numerous plasmablast-derived immunoglobulin lineages persisted in the bone marrow >8 months after immunization, including in the CD138+ long-lived plasma cell compartment. These findings identify a cellular linkage for the development of sustained Env-specific Abs following vaccination in humans. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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