PCR نیمهآشیانهای برای تشخیص آلودگی نئوسپورا کنینوم در گاوها
| Autor: | غلامرضا حبیبی, ر. هاشما فشارکی, ا. صدربزاز, س. بزرگی, ن. بردبار |
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| Jazyk: | English<br />French |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Archives of Razi Institute, Vol 59, Iss 2, Pp 55-64 (2005) |
| Druh dokumentu: | article |
| ISSN: | 0365-3439 2008-9872 |
| DOI: | 10.22092/ari.2005.103813 |
| Popis: | Neospora caninum, an apicomplexan protozoan, is regarded as a major cause of abortion and stillbirth in cattle in countries world-wide. The ability to detection N.caninum in tissue samples can be a useful detection diagnostic tool for use in the study of the pathogenicity, immunoprophylaxis, and treatment of Neospora infection. However, molecular biology is one of the most sensitive tools for detecting protozoa in infected tissue samples. Specific semi-nested PCR was designed based on specific ITS1 and 5.8S rRNA genomic DNA for detection of parasite in infected tissues. The designed PCR detected four of six aborted fetal brain samples infected by N.caninum. Our results revealed that PCR with selected primers gave a 357bp product in examined samples and confirmed the presence of N.caninum DNA in infected fetal brains. This is the first report that demonstrated the reliability of PCR-based assay to identify N.caninum infection in Iran. |
| Databáze: | Directory of Open Access Journals |
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