Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing

Autor: Shanshan Cheng, Yuqian Shen, Zi Chao Zhang, Junhai Han
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 3, Pp 100679- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100679
Popis: Summary: Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an interfering peptide into the adult mouse hippocampus. The delivered peptide disrupts the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2, resulting in impaired hippocampal mGluR-LTD and mGluR-LTD-associated behaviors.For complete details on the use and execution of this protocol, please refer to Shen et al. (2021).
Databáze: Directory of Open Access Journals