Which resolution?

Autor: Colin Nave
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: IUCrJ, Vol 10, Iss 5, Pp 603-609 (2023)
Druh dokumentu: article
ISSN: 2052-2525
20522525
DOI: 10.1107/S205225252300698X
Popis: The relationship between the contrast to noise ratio and intensity based cross-correlation coefficients for both protein crystallography and X-ray imaging are compared. It is concluded that, for protein crystallography at near atomic resolution, the intensity based cross-correlation coefficients give a reasonable indication of the quality of the corresponding electron density. For X-ray imaging of biological materials such as cells and soft tissue, the wide range of contrast of the features means that intensity based correlation coefficients can give a poor indication of the interpretability of an image. Rather than the term resolution, it is the contrast to noise ratio for a feature of interest at the relevant spatial frequency that is more relevant. Additional metrics are required to describe the quality of an image, and these are discussed.
Databáze: Directory of Open Access Journals