In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells

Autor: Alona Zlatska, Inna Gordiienko, Roman Vasyliev, Dmitriy Zubov, Olga Gubar, Anzhela Rodnichenko, Anton Syroeshkin, Igor Zlatskiy
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: The Scientific World Journal, Vol 2018 (2018)
Druh dokumentu: article
ISSN: 2356-6140
1537-744X
DOI: 10.1155/2018/5454367
Popis: In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.
Databáze: Directory of Open Access Journals
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