Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds
| Autor: | M. Cagnazzo, S. Braglia, R. Davoli |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Italian Journal of Animal Science, Vol 4, Iss 2s, Pp 91-93 (2010) |
| Druh dokumentu: | article |
| ISSN: | 1594-4077 1828-051X |
| DOI: | 10.4081/ijas.2005.2s.91 |
| Popis: | The quantitative real time-PCR (QRT-PCR) is a very sensitive method used to quantify mRNA level in gene expression analysis. Combining amplification, detection and quantification in a single step, allows a more accurate measurement compared to the traditional PCR end point analysis (Pfaffl, 2001; Bustin, 2002). |
| Databáze: | Directory of Open Access Journals |
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