Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study

Autor: V. A. Karachevtsev, N. T. Kartel, L. V. Ivanov, A. N. Lyapunov, O. A. Nardid, Ya. O. Cherkashina, A. Yu. Ivanov
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Spectroscopy, Vol 2019 (2019)
Druh dokumentu: article
ISSN: 2314-4920
2314-4939
DOI: 10.1155/2019/8083207
Popis: The change in the microviscosity of erythrocyte membranes and the proteins in blood plasma after graphene oxide addition is studied by the ESR spectroscopy exploiting two spin probes with different lipophilic components in the structures. Experiments with charged spin probe 2 embedded into the erythrocyte membrane showed that the introduction of graphene oxide in small concentrations (∼70 μg/ml) into a suspension of erythrocytes did not lead to significant changes in the microviscosity of their membranes. Correlation times of hydrophobic spin probe 1 adsorbed to hydrophobic pockets of plasma proteins demonstrate a gradual slowdown at the graphene oxide injection into blood plasma that indicates a small deformation of the hydrophobic cavity of protein at the adsorption. However, this protein binding with graphene oxide does not cause the displacement of the spin probe from their hydrophobic cavities, which is evidence about small changes in the protein secondary structure. The obtained results indicate the insignificant cytotoxicity effect of small concentrations of graphene oxide for erythrocytes and blood plasma.
Databáze: Directory of Open Access Journals
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