| Autor: |
Feirong Yuan, Yaoming Yuan, Zhixin Li, Alessandra Gentile, Ziniu Deng |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Horticultural Plant Journal, Vol 2, Iss 3, Pp 126-132 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2468-0141 |
| DOI: |
10.1016/j.hpj.2016.08.001 |
| Popis: |
Transgenic citrange lines with rolABC genes behave rosette branching and extreme dwarfing. To explore the regulatory mechanism of plant hormones in axillary shoot growth of transgenic citrange, a full length cDNA of CPMAX2 was cloned from citrange [Citrus sinensis (L.) Osb × Poncirus trifoliate (L.) Raf.] by RT-PCR in this study. The expression of CPMAX2 was detected in axillary tender leaves of 3 transgenic citrange lines and the wild type. Additionally, we constructed an over-expression vector CPMAX2-pCAMBIA1301 for further study. The results showed that the cDNA sequence and its putative peptide sequence shared 99.66% and 99.14% of identity with its Citrus sinensis ortholog MAX2. The deduced amino acid sequence contained putatively one F-box and two LRR repeat domains that are highly conserved in MAX2 genes. CPMAX2 was obviously down-expressed in tender leaves of 3 transgenic citrange lines compared with the wild type. The results suggest CPMAX2 play an important role in regulating the rosette shoot growth in transgenic citrange with rolABC genes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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