Regulation of connective tissue development by calcium channel blocking agents in adhesion process
| Autor: | S. V. Skalskii, T. F. Sokolova, S. K. Zyryanov |
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| Jazyk: | ruština |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Фармакокинетика и Фармакодинамика, Vol 0, Iss 4, Pp 46-50 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2587-7836 2686-8830 |
| Popis: | It has been studied the pharmacological effects of calcium channel blocking agents (CCBA), the derivatives of phenylalkilamin (verapamil), benzothiazepine (diltiazem), dihydropyridine (nifedipine) at a concentration of 0,1 mg/ml of the culture medium in the regulation of connective tissues development in the process of peritoneal adhesion. The experiments were carried out on 89 non-native white rats weighing 230-240 g on the 10th day after hemoperitoneum simulation in the primary culture of peritoneal fibroblasts and macrophages. It was specified that the development of peritoneal adhesion was accompanied by excessive proliferative activity of the cells studied with the enhancement of the synthesis of protein-bound oxyproline and hyaluronic acid, the pro-inflammatory cytokines, such as TNF-α and IL-1. Verapamil (0,1 mg/ml) suppressed the proliferation of fibroblasts, prevented the transformation of monocytes into macrophages, reduced the excess production of hydroxyproline and hyaluronic acid, and reduced the synthesis of pro-inflammatory cytokines. Diltiazem (0,1 mg/ml) had a normalizing effect on the functional activity of peritoneal fibroblasts and macrophages, the excess production of the intercellular matrix and cytokines, but the effect was less significant (on average 35,5 %, p |
| Databáze: | Directory of Open Access Journals |
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