Plant regeneration of Rhabdadenia Ragonesei (Apocynaceae) by in vitro culturing of leaf explants
| Autor: | Eduardo Flachsland, Aurelio Schinini, Graciela Terada, Graciela Furst, Luis A. Mroginkski |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Bonplandia, Vol 14, Iss 1-2, Pp 91-96 (2005) |
| Druh dokumentu: | article |
| ISSN: | 0524-0476 1853-8460 |
| Popis: | Plants of Rhabdadenia Ragonesei Woodson (Apocynaceae) were regenerated in vitro from leaves explants. The procedure employed includes: 1) Surface sterilization of leaves by immersion in 70% ethanol (10 s) followed by 1,1%NaOCl (15 min) and three wash with sterile distilled water. 2) Callus and buds induction by culture on Murashige and Skoog medium (MS) + 3 mg/L benzyladenine (BAP). 3) Subculture of callus and buds on MS + 1 mg/L BAP, and 4) Rooting on MS + 0.5 mg/L naftalenacetic acid |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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