New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA
| Autor: | Nathan Wales, Christian Carøe, Marcela Sandoval-Velasco, Cristina Gamba, Ross Barnett, José Alfredo Samaniego, Jazmín Ramos Madrigal, Ludovic Orlando, M. Thomas P. Gilbert |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 59, Iss 6, Pp 368-371 (2015) |
| Druh dokumentu: | article |
| ISSN: | 00011436 1940-9818 0736-6205 |
| DOI: | 10.2144/000114364 |
| Popis: | An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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