Comparison of urinary proteome in the first two days after mating in male rats

Autor: Haitong Wang, Chenyang Zhao, Youhe Gao
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Advances in Biomarker Sciences and Technology, Vol 6, Iss , Pp 166-173 (2024)
Druh dokumentu: article
ISSN: 2543-1064
DOI: 10.1016/j.abst.2024.08.003
Popis: Objective: To explore whether differences between male rats on the next day of mating and on the day of mating can be reflected by the urine proteome. Methods: Urine samples were collected from male Sprague-Dawley rats on the day of mating and the next day of mating. Urine samples were analysed by the label-free quantitative proteomics technique of high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Differential proteins of the urine proteome were analysed for protein function and biological pathways. Results: 54 differential proteins were identified by comparing the urine proteome of rats on the next day of mating with that on the day of mating, and nearly two-thirds of the differential proteins were related to spermatogenesis. Conclusions: The urine proteome has the potential to reflect spermatogenesis without interfering with it.
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