| Autor: |
Francesc Hernández-Gras, Rocco Petrizzo, Irini Pateraki, Marta Renato, Maxime Angaman, Joaquín Azcón-Bieto, Albert Boronat |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 4, Iss 15 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.1192 |
| Popis: |
It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed protocols for the isolation of tomato fruit chromoplasts and the determination of ATP levels (end-point measurements) and ATP synthesis rates (kinetic measurements) in these organelles using bioluminescent luciferin/luciferase based assays. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
