| Autor: |
M.T. Kelley, R.T. Aexel, B.L. Herndon, H.J. Nicholas |
| Jazyk: |
angličtina |
| Rok vydání: |
1969 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 10, Iss 2, Pp 166-174 (1969) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)42663-X |
| Popis: |
Cell-free extracts of adult rat brain incubated with mevalonic acid-2-14C synthesize 14C-labeled nonsaponifiable fractions consisting largely of squalene-14C. If the cofactor concentrations of the incubation medium are adjusted, much of the squalene can be induced to undergo turnover, with a resultant increase in 14C-labeled digitonin-precipitable sterols, which include a small amount of cholesterol.The synthesis of labeled sterols is markedly increased in the presence of Mg++ and depressed by nicotinamide. ATP, NADH, GSH, and glucose-6-phosphate are required for optimal synthesis of digitonin-precipitable material but, unlike Mg++, are not essential.The cofactor-adjusted extracts also synthesize a complex ester mixture containing, in addition to cholesterol-14C, several compounds less polar than cholesterol. The biosynthesis of cholesterol in the extracts is a slow process; at least 12 hr of incubation is required for maximal sterol biosynthesis. A complex mixture of hydrocarbons accompanies squalene in the incubated extracts. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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