| Autor: |
Wei Guo, Zhuoqi Liu, Jiaqi Yu, Jing Lü, Xiaoguo Jiao, Lan Kong, Chunxiao Yang, Huipeng Pan |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Horticultural Plant Journal, Vol 7, Iss 2, Pp 142-148 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2468-0141 |
| DOI: |
10.1016/j.hpj.2020.08.003 |
| Popis: |
Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three male H. vigintioctopunctata libraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, and de novo assembly generated 85 206 unigenes, with N50 of 1 311 bp and average length of 763 bp. We found 5 002 genes highly expressed in males and 2 179 genes highly expressed in females. Expression profiles of six unigenes specific to females, six unigenes specific to males, and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR; results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis. Using all unigenes as references, we identified 6 657 putative simple sequence repeats. The transcriptome sequences and gene expression profiles of female and male H. vigintioctopunctata help explore genetic mechanisms of sexual dimorphism. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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