A specific combined long-chain polyunsaturated fatty acid supplementation reverses fatty acid profile alterations in a mouse model of chronic asthma

Autor: D. Fussbroich, K. Zimmermann, A. Göpel, O. Eickmeier, J. Trischler, S. Zielen, R. Schubert, C. Beermann
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Lipids in Health and Disease, Vol 18, Iss 1, Pp 1-10 (2019)
Druh dokumentu: article
ISSN: 1476-511X
02317915
DOI: 10.1186/s12944-018-0947-6
Popis: Abstract Background The immune-modulating potential of long-chain polyunsaturated fatty acids (LCPUFAs) based on their conversion into lipid mediators in inflammatory situations has been proven by several studies. Respecting the immune-modulative role of lipid mediators in bronchoconstriction, airway inflammation and resolution of inflammatory processes, LCPUFAs play an important role in asthma. To design a disease-specific and most beneficial LCPUFA supplementation strategy, it is essential to understand how asthma alters LCPUFA profiles. Therefore, this study characterizes the alterations of LCPUFA profiles induced by allergic asthma. In addition, this study explores whether a simple eicosapentaenoic acid (EPA) alone or a specific combined LCPUFA supplementation could restore imbalanced LCPUFA profiles. Methods Mice were sensitized with a daily dose of 40 μg house dust mite (HDM)-extract in a recall model and fed with either normal diet, EPA or a specific combined (sc)-LCPUFA supplementation containing EPA, docosahexaenoic acid (DHA), γ -linolenic acid (GLA) and stearidonic acid (SDA) for 24 days. After recall with HDM, mice were sacrificed and blood and lung tissue were collected. Fatty acid profiles were determined in plasma, blood cells and lung cells of asthmatic mice by capillary gas-chromatography. Results In lung cells of asthmatic mice, arachidonic acid (AA, p
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