Identification of Sphaerulina azaleae on Korean Azalea in Korea Based on Morphological Characteristics and Multilocus Sequence Typing

Autor: In-Young Choi, Young-Joon Choi, Kui-Jae Lee, Ho-Jong Ju, Seong-Wan Cho, Hyeon-Dong Shin
Jazyk: English<br />Korean
Rok vydání: 2020
Předmět:
Zdroj: 한국균학회지, Vol 48, Iss 3, Pp 329-335 (2020)
Druh dokumentu: article
ISSN: 0253-651X
2383-5249
DOI: 10.4489/KJM.20200032
Popis: From 2008 to 2017, Korean azalea (Rhododendron yedoense f. poukhanense) showing angular, necrotic leaf spots were found in Jeju and Hongcheon, Korea. The lesions occurred frequently, detracting from the beauty of the glossy green leaves of the plant and causing premature defoliation. Therefore, to identify the fungus associated with the lesions, morphological characterization and molecular phylogenetic analysis of actin (Act), translation elongation factor 1-alpha (EF), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II encoding the second largest subunit (RPB2) of the two representative isolates were performed. The phylogenetic tree inferred from the neighbor-joining method showed the isolates clustering in the Sphaerulina azaleae group. Therefore, the fungus associated with the angular leaf spots on the Korean azalea was identified as Sphaerulina azaleae.
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