| Autor: |
Ngan Thi Phuong Le, Trang Thi Phuong Phan, Hanh Thi Thu Phan, Tuom Thi Tinh Truong, Wolfgang Schumann, Hoang Duc Nguyen |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Biotechnology Reports, Vol 35, Iss , Pp e00754- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2215-017X |
| DOI: |
10.1016/j.btre.2022.e00754 |
| Popis: |
The influence of fusion tags to produce recombinant proteins in the cytoplasm of Bacillus subtilis is not well-studied as in E. coli. This study aimed to investigate the influence of His-tags with different codons on the protein production levels of the high expression gene (gfp+) and low expression gene (egfp) in the cytoplasm of B. subtilis cells. We used three different N-terminal His-tags, M-6xHis, MRGS-8xHis and MEA-8xHis, to investigate their effects on the production levels of GFP variants under the control of the Pgrac212 in B. subtilis. The fusions of His-tags with GFP+ caused a reduction compared to the construct without His-tag. When three His-tags fused with egfp, the EGFP production levels were significantly increased up to 3.5-, 12-, and 15-fold. This study suggested that His-tag at the N-terminus could enhance the protein production for the low expression gene and reduce that of the high expression gene in B. subtilis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
