Modulation of lipid peroxidation and energy metabolism in the gastric mucosa as a mechanism of antiulcer activity of placental cryoextract in the healing of stress-induced ulcers
| Autor: | I.V. Koshurba, F.V. Hladkykh, M.O. Chyzh |
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| Jazyk: | English<br />Ukrainian |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Gastroenterologìa, Vol 56, Iss 3, Pp 149-155 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2308-2097 2518-7880 |
| DOI: | 10.22141/2308-2097.56.3.2022.503 |
| Popis: | Background. Peptic ulcer disease has a leading place in the overall structure of digestive diseases: its prevalence is 6.00–10.0 % of the population in developed countries, and mortality ranges from 6 to 9.7 per 100 thousand population. An important etiological factor of this pathology is the neuropsychological background, primarily stress, which under repeated exposure becomes the initiating factor of physiological imbalance between the elements of aggression and protection of the gastric mucosa. The purpose was to establish the mechanisms of the protective activity of placental cryoextract based on the biochemical indicators of lipid peroxidation and antioxidant protection in the gastric mucosa on a model of stress-induced erosive-ulcerative damage. Materials and methods. Studies were performed on 28 nonlinear laboratory male rats weighing 200–220 g. Stress-induced gastric ulcer was modeled under water-immobilization stress in rats according to the K.Y. Takagi et al. In the gastric mucosa, the content of reactants with thiobarbituric acid was determined spectrophotometrically by the method of T. Asakawa et al., catalase activity — spectrophotometrically by the method of M.A. Korolyuk et al., the content of reduced glutathione — spectrophotometrically by the method of E.D. Beutler et al., the level of adenyl nucleotides was determined using chromatographic method. Energy charge was calculated by D.E. Atkinson equation. Results. The prophylactic five-day use of placental cryoextract led to a decrease in the severity of stress-induced lipid peroxidation and energy imbalance in the gastric mucosa. Thus, it was found that rats who received placental cryoextract had a statistically significant (p |
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