Ceratonia siliqua L. pod Effects on Viability Gene Expression of Endometrial Mesenchymal Stromal/Stem Cells Isolated from Women with Endometriosis-Associated Infertility

Autor: Zahra Khodabandeh, Bahia Namavar Jahromi, Atefe Hashemi, Kamran Hessami, Iman jamhiri, Shahrokh Zare, Parmis Badr, Aida Iraji, Tahere Poordast, Neda Baghban, Arezoo Khoradmehr, Nadiar Maratovich Mussin, Asset Askerovich Kaliyev, Yerbolat Maratovich Iztleuov, Reza Shirazi, Mahdi Mahdipour, Shabnam Bakhshalizadeh, Farhad Rahmanifar, Nazanin Jafari, Nader Tanideh, Amin Tamadon
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: International Journal of Fertility and Sterility, Vol 18, Iss 4, Pp 391-403 (2024)
Druh dokumentu: article
ISSN: 2008-076X
2008-0778
DOI: 10.22074/ijfs.2023.2007228.1496
Popis: Background: This study aims to investigate the effects of carob (Ceratonia siliqua L.) pod extract (CPE) on theviability of human endometrial mesenchymal stromal/stem cells (EnMSCs) and its impact on mRNA and protein expressionsof DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), histone deacetylase 1 (HDAC1), matrixmetalloproteinase-2 (MMP2), and cyclooxygenase-2 (COX-2) in endometriotic patients.Materials and Methods: In this experimental study, EnMSCs were derived from endometrium of patients with ovarianendometrioma (OMA-EnMSCs group) and deep infiltrative endometriosis (DIE) samples of 10 endometriosisassociatedinfertility (EAI) women (E-EnMSCs group) and compared to EnMSCs derived from the endometrium ofan endometriosis-free, normal woman as the control group (C-EnMSCs). The metabolic activity of the control andcase groups were evaluated by treating them with different concentrations of CPE. Cell viability was analysed byMTT. Real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to evaluatethe expression of specific genes at the mRNA and protein levels, respectively.Results: Treatment with 0.8 and 2 μg/mL of CPE downregulated COX-2 and HDAC1 in the E-EnMSC groupcompared to the C-EnMSCs group. Treatment with 0.8 μg/mL of CPE also decreased MMP2 and DNMT3B geneexpressions. The COX-2 and DNMT3A genes were significantly upregulated after treatment with 2 μg/mL of CPE.Expressions of the COX-2, HDAC1, DNMT1, DNMT3A, and DNMT3B peptides decreased in the all three groups after treatment with 0.8 and 2 μg/mL of CPE. Gas chromatography-mass spectroscopy (GC-MS) analysis of CPEidentified 14 bioactive compounds. Molecular docking showed the best position of each bioactive compound on thedifferent target proteins that are involved in the process of apoptosis in EnMSCs.Conclusion: In vitro and in silico analyses of CPE bioactive compounds show that they may downregulate the cellinflammatory pathway involved in the pathophysiology of endometriosis.
Databáze: Directory of Open Access Journals
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