| Popis: |
Background. The NLP-type effectors of Phytophthora capsici are involved in the establishment of the disease and necrosis in solanaceous plants such as Capsicum chinense, a crop that has gained great interest worldwide, and is susceptible to oomycete infection. Objective. To evaluate the expression of 18 NLP-type genes during the interaction of Phytophthora capsici against Capsicum chinense, treated with 2-chloroethyl phosphonic acid (ethephon). Methodology. Capsicum chinense plants were sprayed with ethephon at 0, 2.5, 5 and 10 mM/ml and inoculated with P. capsici mycelium. Gene expression analyzes by RT-PCR were carried out for NLP-type genes. Results. In plants sprayed with 5 mM/ml ethephon, incised and infected, the necrosis transcripts reported were: Pcnpp ART-1, Pnnp BRT-2, Pcnpp FRT-3, Pcnpp GRT-7, Pcnpp LRT-8. While no transcripts were observed in genes Pcnpp KRT-4, Pcnpp HRT-5, Pcnpp JRT-6, Pcnpp MRT-9, Pcnpp RRT-12, Pcnpp TRT-13, Pcnpp URT-14, Pcnpp XRT-15, Pcnpp YRT -16 and Pcnpp SRT-17. With the plants sprayed with phosphate buffer, without incision, the transcripts reported were: Pcnpp BRT-2, Pcnpp LRT-8. Implications. Ethephon induces tolerance to omycete. Conclusions. There are differences in gene expression between plants sprayed with ethephon, with incision and infected with P. capsici compared to plants sprayed and without incision. The transcript that was expressed in both groups of plants was BRT-2 and LRT-8, it is concluded that these genes are of greater relevance for a successful infection in this interaction. |
