Influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction
| Autor: | David MARTÍN-HIDALGO, Beatriz MACÍAS-GARCÍA, Lauro GONZÁLEZ-FERNÁNDEZ |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | The Journal of Reproduction and Development, Vol 68, Iss 1, Pp 68-73 (2021) |
| Druh dokumentu: | article |
| ISSN: | 0916-8818 1348-4400 |
| DOI: | 10.1262/jrd.2021-075 |
| Popis: | We aimed to analyze the influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction. When spermatozoa were incubated at 100 or 200 mill/ml, significant increases in protein tyrosine phosphorylation in the p32 protein were observed, compared to those at 50 mill/ml. In addition, sperm concentration-dependent increases were observed in plasma membrane lipid disorganization (50 mill/ml vs. 200 mill/ml), induction of the acrosome reaction (50 mill/ml vs. 100 mill/ml and 200 mill/ml), and sperm viability (50 mill/ml vs. 100 mill/ml and 200 mill/ml). Our data indicate that an increase in sperm concentration stimulates the induction of capacitation and acrosome reaction in boars. |
| Databáze: | Directory of Open Access Journals |
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