Platelet-rich plasma activates AKT signaling to promote wound healing in a mouse model of radiation-induced skin injury

Autor: Janet Lee, Hyosun Jang, Sunhoo Park, Hyunwook Myung, Kyuchang Kim, Hyewon Kim, Won-Suk Jang, Sun-Joo Lee, Jae Kyung Myung, Sehwan Shim
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Translational Medicine, Vol 17, Iss 1, Pp 1-10 (2019)
Druh dokumentu: article
ISSN: 1479-5876
DOI: 10.1186/s12967-019-2044-7
Popis: Abstract Background The skin is impacted by every form of external radiation therapy. However, effective therapeutic options for severe, acute radiation-induced skin reactions are limited. Although platelet-rich plasma (PRP) is known to improve cutaneous wound healing, its effects in the context of high-dose irradiation are still poorly understood. Methods We investigated the regenerative functions of PRP by subjecting the dorsal skin of mice to local irradiation (40 Gy) and exposing HaCaT cells to gamma rays (5 Gy). The cutaneous benefits of PRP were gauged by wound size, histologic features, immunostains, western blot, and transepithelial water loss (TEWL). To assess the molecular effects of PRP on keratinocytes of healing radiation-induced wounds, we evaluated AKT signaling. Results Heightened expression of keratin 14 (K14) was documented in irradiated HaCaT cells and skin tissue, although the healing capacity of injured HaCaT cells declined. By applying PRP, this capacity was restored via augmented AKT signaling. In our mouse model, PRP use achieved the following: (1) healing of desquamated skin, acutely injured by radiation; (2) activated AKT signaling, improving migration and proliferation of K14 cells; (3) greater expression of involucrin in keratin 10 cells and sebaceous glands; and (4) reduced TEWL, strengthening the cutaneous barrier function. Conclusions Our findings indicate that PRP enhances the functions of K14 cells via AKT signaling, accelerating the regeneration of irradiated skin. These wound-healing benefits may have merit in a clinical setting.
Databáze: Directory of Open Access Journals
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