Comparison of Transforming Growth Factor 1 (TGF-β1) Expression in Various Lysate Platelet-Rich Fibrin (L-PRF) Concentrations on Human Dental Pulp Stem Cell Differentiation
| Autor: | Muhammad Zaal Haq, Anggraini Margono, R. A. H. Nilakesuma Djauharie, Dini Asrianti, Indra Topanesa, Indah Julianto |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada, Vol 21 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1519-0501 1983-4632 |
| Popis: | Objective: To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material and Methods: hDPSC cell cultures were subjected to serum starvation by reducing FBS levels in the hDPSC culture media. Lysate PRF was obtained from the PRF gel, which was then incubated at 4°C for 24 h. The supernatant was dried, transferred to a 2-ml Eppendorf tube, and stored at −20°C. The evaluation of TGF-β1 expression in 1%, 5%, 10%, and 25% L-PRF samples and 10% FBS (control) during the process of hDPSC differentiation was quantified using an ELISA reader on day 7. The expression of TGF-β1 was subjected to a one-way ANOVA test, followed by Bonferroni’s post hoc test with significant values (p |
| Databáze: | Directory of Open Access Journals |
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