| Autor: |
Haoyu Ge, Jie Qiao, Jiahao Zheng, Hao Xu, Ruishan Liu, Junhui Zhao, Ruyan Chen, Chenyu Li, Xiaobing Guo, Beiwen Zheng |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Annals of Clinical Microbiology and Antimicrobials, Vol 22, Iss 1, Pp 1-9 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1476-0711 |
| DOI: |
10.1186/s12941-023-00577-z |
| Popis: |
Abstract Background Despite the global prevalence of Klebsiella pneumoniae Carbapenemase (KPC)-type class A β-lactamases, occurrences of KPC-3-producing isolates in China remain infrequent. This study aims to explore the emergence, antibiotic resistance profiles, and plasmid characteristics of bla KPC-3-carrying Pseudomonas aeruginosa. Methods Species identification was performed by MALDI-TOF-MS, and antimicrobial resistance genes (ARGs) were identified by polymerase chain reaction (PCR). The characteristics of the target strain were detected by whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST). Plasmids were analyzed by S1-nuclease pulsed-field gel electrophoresis(S1-PFGE), Southern blotting and transconjugation experiment. Results Five P. aeruginosa strains carrying bla KPC-3 were isolated from two Chinese patients without a history of travelling to endemic areas. All strains belonged to the novel sequence type ST1076. The bla KPC-3 was carried on a 395-kb IncP-2 megaplasmid with a conserved structure (IS6100-ISKpn27-bla KPC-3-ISKpn6-korC-klcA), and this genetic sequence was identical to many plasmid-encoded KPC of Pseudomonas species. By further analyzing the genetic context, it was supposed that the original of bla KPC-3 in our work was a series of mutation of bla KPC-2. Conclusions The emergence of a multidrug resistance IncP-2 megaplasmid and clonal transmission of bla KPC-3-producing P. aeruginosa in China underlined the crucial need for continuous monitoring of bla KPC-3 for prevention and control of its further dissemination in China. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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