Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
| Autor: | Valessa Lunkes Ely, Letícia Trevisan Gressler, Fernando Jonas Sutili, Márcio Garcia Ribeiro, Mateus Matiuzzi da Costa, Agueda Castagna de Vargas, Sônia de Avila Botton |
|---|---|
| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 49, Iss 2 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/0103-8478cr20180574 |
| Popis: | ABSTRACT: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|