DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina
| Autor: | Sofrenić Ivana V., Anđelković Boban D., Vujisić Ljubodrag, Novaković Miroslav, Knežević Aleksandar Z., Stanković Miroslava B., Milosavljević Slobodan M., Tešević Vele V. |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of the Serbian Chemical Society, Vol 86, Iss 9, Pp 809-817 (2021) |
| Druh dokumentu: | article |
| ISSN: | 0352-5139 1820-7421 |
| DOI: | 10.2298/JSC210401039S |
| Popis: | Eleven 31-methylenlanostane triterpenoids, i.e., seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina, were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds showed a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in the concentration dependent manner, with the concentration of 2.0 μg mL-1 being the most effective – with increase of the concentration the activity slightly decreases. The structure–activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than 8-analogues. It was also demonstrated that the DNA protective activities within the 8-lanostane-26-oic acid group are affected by the substitution in position 3 pattern. In the 8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but it is still comparable to that of amifostine. |
| Databáze: | Directory of Open Access Journals |
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