| Autor: |
Sara Ruiz-Díaz, Rosane Mazzarella, Paula Navarrete-López, Raúl Fernández-González, Celia de Frutos, María Maroto, Claudia Cucala, Paula Beltrán-Breña, Marta Lombó, Dimitrios Rizos, Alfonso Gutiérrez-Adán |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Journal of Animal Science and Biotechnology, Vol 14, Iss 1, Pp 1-11 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2049-1891 |
| DOI: |
10.1186/s40104-022-00810-3 |
| Popis: |
Abstract Background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation (SDF) in mice, humans, and stallions. This study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI. Methods We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of caffeine. In these conditions, sperm selection was achieved, obtaining a net thermotaxis of 3.6%. Subsequently, we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay, and we evaluated the size of the sperm head using Hemacolor® staining with Motic Images Plus 3 software. Additionally, migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured (IVM) oocytes by ICSI, a very inefficient procedure in cattle that is only successful when the oocyte is artificially activated. Results The results showed lower SDF (χ², P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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