| Autor: |
Abed Alkarem Abu Alhaija, Imtiaz Nisar Lone, Esin Ozkuru Sekeroglu, Tugce Batur, Dimitar Angelov, Stefan Dimitrov, Ali Hamiche, Elif Nur Firat Karalar, Muhammed Erdem Ercan, Tamer Yagci, Hani Alotaibi, Muhammed Kasim Diril |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
FEBS Open Bio, Vol 14, Iss 2, Pp 309-321 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2211-5463 |
| DOI: |
10.1002/2211-5463.13750 |
| Popis: |
The linker histone H1 C‐terminal domain (CTD) plays a pivotal role in chromatin condensation. De novo frameshift mutations within the CTD coding region of H1.4 have recently been reported to be associated with Rahman syndrome, a neurological disease that causes intellectual disability and overgrowth. To investigate the mechanisms and pathogenesis of Rahman syndrome, we developed a cellular model using murine embryonic stem cells (mESCs) and CRISPR/Cas9 genome engineering. Our engineered mES cells facilitate detailed investigations, such as H1‐4 dynamics, immunoprecipitation, and nuclear localization; in addition, we tagged the mutant H1‐4 with a photoactivatable GFP (PA‐GFP) and an HA tag to facilitate pulldown assays. We anticipate that these engineered cells could also be used for the development of a mouse model to study the in vivo role of the H1‐4 protein. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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