| Autor: |
Jirarat Songsri, Moragot Chatatikun, Sueptrakool Wisessombat, Wanida Mala, Preeda Phothaworn, Wilaiwan Senghoi, Wilawan Palachum, Wetpisit Chanmol, Nuchpicha Intakhan, Sirithip Chuaijit, Pakpoom Wongyikul, Phichayut Phinyo, Kenshi Yamasaki, Anchalee Chittamma, Wiyada Kwanhian Klangbud |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Journal of Infection and Public Health, Vol 17, Iss 7, Pp 102438- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1876-0341 |
| DOI: |
10.1016/j.jiph.2024.04.022 |
| Popis: |
Background: Burkholderia pseudomallei, a Gram-negative pathogen, causes melioidosis. Although various clinical laboratory identification methods exist, culture-based techniques lack comprehensive evaluation. Thus, this systematic review and meta-analysis aimed to assess the diagnostic accuracy of culture-based automation and non-automation methods. Methods: Data were collected via PubMed/MEDLINE, EMBASE, and Scopus using specific search strategies. Selected studies underwent bias assessment using QUADAS-2. Sensitivity and specificity were computed, generating pooled estimates. Heterogeneity was assessed using I2 statistics. Results: The review encompassed 20 studies with 2988 B. pseudomallei samples and 753 non-B. pseudomallei samples. Automation-based methods, particularly with updating databases, exhibited high pooled sensitivity (82.79%; 95% CI 64.44–95.85%) and specificity (99.94%; 95% CI 98.93–100.00%). Subgroup analysis highlighted superior sensitivity for updating-database automation (96.42%, 95% CI 90.01–99.87%) compared to non-updating (3.31%, 95% CI 0.00–10.28%), while specificity remained high at 99.94% (95% CI 98.93–100%). Non-automation methods displayed varying sensitivity and specificity. In-house latex agglutination demonstrated the highest sensitivity (100%; 95% CI 98.49–100%), followed by commercial latex agglutination (99.24%; 95% CI 96.64–100%). However, API 20E had the lowest sensitivity (19.42%; 95% CI 12.94–28.10%). Overall, non-automation tools showed sensitivity of 88.34% (95% CI 77.30–96.25%) and specificity of 90.76% (95% CI 78.45–98.57%). Conclusion: The study underscores automation's crucial role in accurately identifying B. pseudomallei, supporting evidence-based melioidosis management decisions. Automation technologies, especially those with updating databases, provide reliable and efficient identification. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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