| Autor: |
Thiago Seike Nakahara, Vinicius Miessler de Andrade Carvalho, Mateus Augusto de Andrade Souza, Guilherme Ziegler Trintinalia, Fabio Papes |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 1, Iss 3, Pp 100153- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100153 |
| Popis: |
Summary: This protocol combines fluorescent in situ hybridization and immunostaining to simultaneously detect, in histological sections from the same animal, subpopulations of neurons activated after two episodes of sensory stimulation. It allows the identification of groups of cells singly activated by either stimulus or co-activated by both stimuli. Our method results in nuclear staining for c-Fos mRNA and c-Fos protein, allowing better spatial and temporal resolution than previously published protocols, although it requires quick brain fixation.For complete details on the use and execution of this protocol, please refer to Carvalho et al. (2015, 2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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