| Autor: |
Yang Peng, Wei-jie Guan, Kai Sen Tan, Zhenchao Zhu, Zhuo Chen, Haiyu Hong, Zhaoni Wang, Tengfei Tian, Xiaoxue Zi, Yew Kwang Ong, Mark Thong, Li Shi, Qintai Yang, Qianhui Qiu, De-Yun Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Allergy, Asthma & Clinical Immunology, Vol 14, Iss 1, Pp 1-9 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1710-1492 |
| DOI: |
10.1186/s13223-018-0296-z |
| Popis: |
Abstract Background Upper airway inflammatory diseases are associated with abnormal expression of nasal epithelial forkhead-box J1 (FOXJ1) which regulates motile cilia formation. We sought to investigate whether aberrant FOXJ1 localizations correlate with the disease severity and the co-existence of allergic rhinitis (AR) or asthma in patients with nasal polyps (NPs). Methods We elucidated localization patterns of FOXJ1 by performing immunofluorescence assays in nasal specimens and cytospin samples from controls and patients with NPs. We also assayed mRNA expression levels of FOXJ1 by using quantitative real-time polymerase chain reaction. Four localization patterns [normal (N), intermediate (I), mislocalization (M), and absence (A)] were defined. A semi-quantitative scoring system was applied for demonstrating FOXJ1 localization in five areas per paraffin section, with individual sections being scored between 0 and 2. Results FOXJ1 localization score was significantly higher in samples from NPs than in controls (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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