A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W

Autor: Biao Huang, Huixin Yu, Jiandong Bao, Manda Zhang, William L Green, Sing-Yung Wu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Biomarker Insights, Vol 13 (2018)
Druh dokumentu: article
ISSN: 1177-2719
11772719
DOI: 10.1177/1177271918757484
Popis: Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje