Prevention against diffuse spinal cord astrocytoma: can the Notch pathway be a novel treatment target?
| Autor: | Jian-jun Sun, Zhen-yu Wang, Ling-song Li, Hai-yan Yu, Yong-sheng Xu, Hai-bo Wu, Yi Luo, Bin Liu, Mei Zheng, Jin-long Mao, Xiao-hui Lou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Alzheimer′s disease
amyloid-β astrocytes Ca 2+ calcilytic calcium-sensing receptor nitromemantine NPS 2143 α7-nicotinic acetylcholine receptor nerve regeneration spinal cord injury surgical decompression tumor necrosis factor α cell apoptosis neurological function neural regeneration contusion Nogo-A axon growth immunohistochemistry fluorescent quantitative PCR Schwann cells cell transplantation edaravone motor function electrophysiological function electroacupuncture intervertebral disc blood circulation inflammation neuroprotection neurons NSFC grants astrocytoma mice immunodeficiency (BALB/c) mice Notch nestin glial fibrillary acidic protein CD133 spinal cord brain MRI Neurology. Diseases of the nervous system RC346-429 |
| Zdroj: | Neural Regeneration Research, Vol 10, Iss 2, Pp 244-251 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.152378 |
| Popis: | This study was designed to investigate whether the Notch pathway is involved in the development of diffuse spinal cord astrocytomas. BALB/c nude mice received injections of CD133 + and CD133− cell suspensions prepared using human recurrent diffuse spinal cord astrocytoma tissue through administration into the right parietal lobe. After 7-11 weeks, magnetic resonance imaging was performed weekly. Xenografts were observed on the surfaces of the brains of mice receiving the CD133 + cell suspension, and Notch-immunopositive expression was observed in the xenografts. By contrast, no xenografts appeared in the identical position on the surfaces of the brains of mice receiving the CD133− cell suspension, and Notch-immunopositive expression was hardly detected either. Hematoxylin-eosin staining and immunohistochemical staining revealed xenografts on the convex surfaces of the brains of mice that underwent CD133 + astrocytoma transplantation. Some sporadic astroglioma cells showed pseudopodium-like structures, which extended into the cerebral white matter. However, it should be emphasized that the subcortex xenograft with Notch-immunopositive expression was found in the fourth mouse received injection of CD133− astrocytoma cells. However, these findings suggest that the Notch pathway plays an important role in the formation of astrocytomas, and can be considered a novel treatment target for diffuse spinal cord astrocytoma. |
| Databáze: | Directory of Open Access Journals |
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