Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers
| Autor: | Ikuma Kasuga, Peter D. Paré, Andrew J. Sandford |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 29, Iss 2, Pp 203-206 (2006) |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 |
| DOI: | 10.1590/S1415-47572006000200003 |
| Popis: | We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods. |
| Databáze: | Directory of Open Access Journals |
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