A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
| Autor: | Raphaël de Wijn, Oliver Hennig, Jennifer Roche, Sylvain Engilberge, Kevin Rollet, Pablo Fernandez-Millan, Karl Brillet, Heike Betat, Mario Mörl, Alain Roussel, Eric Girard, Christoph Mueller-Dieckmann, Gavin C. Fox, Vincent Olieric, José A. Gavira, Bernard Lorber, Claude Sauter |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | IUCrJ, Vol 6, Iss 3, Pp 454-464 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2052-2525 20522525 |
| DOI: | 10.1107/S2052252519003622 |
| Popis: | Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept. |
| Databáze: | Directory of Open Access Journals |
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