Development and validation of an RP-HPLC method for quantification of trans-resveratrol in the plant extracts
| Autor: | Cvetković Žika S., Nikolić Vesna D., Savić Ivan M., Savić-Gajić Ivana M., Nikolić Ljubiša B. |
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| Jazyk: | English<br />Serbian |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Hemijska Industrija, Vol 69, Iss 6, Pp 679-687 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0367-598X 2217-7426 94375887 |
| DOI: | 10.2298/HEMIND140917004C |
| Popis: | New, simple, cost effective, accurate and reproducible RP-HPLC method was developed and validated for the quantification of trans-resveratrol in the extracts of grape exocarp and seeds. The method has proved to be simpler and faster than available methods. Methanol was used as a mobile phase with a flow rate of 1.0 cm3 min-1, while the quantification was effected at 306 nm. The separation was performed at 35°C using a C18 column. The results showed that the peak area response was linear in the concentration range of 1-40 μg cm-3. The values of LOD and LOQ were found to be 0.125 and 0.413 μg cm-3, respectively. The antioxidant activity of the extracts was determined using DPPH assay. The ability of DPPH radicals inhibition decreases in the following order: the extract of grape exocarp > trans-resveratrol standard > the extract of grape seeds. [Projekat Ministarstva nauke Republike Srbije, br. TRp-34012] |
| Databáze: | Directory of Open Access Journals |
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